a listing of all possible genes of an organism.
a relative positioning of genes relative to each other as defined by the genetic nearness of mutations to one another.
an absolute correlation of genes with their locations on chromosomal DNA.
a listing of only the genes that have been identified by mutations.
a positioning of genes on the chromosomal DNA molecule relative to restriction sites in the DNA.
a map that shows the locations of genes that are not subject to mutation and whose analysis is subsequently restricted.
the positioning relative to each other of restriction sites in a DNA molecule.
a listing of all known restriction enzymes and the sequences those enzymes recognize and cleave.
the location of the beginning of the gene of interest.
the orientation of the DNA fragment that codes for the gene of interest.
the exact distance between restriction sites.
restriction enzymes that cleave within the DNA fragment of interest.
digesting the DNA separately with ResA and ResB and comparing the sizes of the resulting DNA fragments.
digesting the DNA simultaeneously with ResA and ResB and comparing the sizes of the resulting DNA fragments.
digesting the DNA both separately with ResA and ResB and simultaeneously with ResA and ResB and comparing the sizes of the resulting DNA fragments.
determining the nucleotide sequence of the DNA fragment, then using a computer program to locate the cleavage sites for ResA and ResB.
small DNA fragments move faster than large DNA fragments in an electric field.
small DNA fragments move slower than large DNA fragments in an electric field.
in an electric field, small DNA fragments move faster through a gel matrix than do large DNA fragments.
in an electric field, small DNA fragments move slower through a gel matrix than do large DNA fragments.
the higher pH at which the polyacrylamide gels must be run.
the higher temperatures at which the agarose gels can be run.
the greater size dimensions of agarose gels.
the smaller matrix pores of the polyacrylamide gel.
direct examination of the gel under the correct wavelength of visible light.
direct examination of the gel under the correct wavelength of ultraviolet light.
staining the DNA in the gel with certain dye compounds, followed by direct examination under the correct wavelength of visible light.
staining the DNA in the gel with intercalating compounds, followed by examination under the correct wavelength of ultraviolet light.
linear relationship between size and mobility.
an approximately logarithimic relationship between size and mobility.
strongly dependent on the concentration of agarose in the gel.
independent of the voltage and current conditions used during electrophoresis.
to provide a set of DNA fragments of known sizes whose mobility can be measured and graphed to provide a standard curve.
practice encouraged by suppliers of DNA standards to ensure continued product sales.
to verify that each gel has performed as expected without error in gel preparation or electrophoresis conditions.
practice that should only be followed when the sizes of unknown DNA fragments on the gel must be determined.
percent GC composition of the DNA molecule.
the size of the DNA molecule.
both the size and conformation of the DNA molecule.
the pH of the electrophoresis buffer in the gel.
the EcoRI and HindIII sites occur very near each other 1000 bp from the left end of the fragment.
the EcoRI and HindIII sites occur very near each other 1000 bp from the right end of the fragment.
nothing can be determined about the restriction map of this DNA fragment.
the restriction map of this fragment contains an EcoRI site 1000 bp from one end of the DNA fragment and a HindIII site 1000 bp from one end of the fragment.
the restriction map of this fragment contains an EcoRI site 1000 bp from one end of the DNA fragment and a HindIII site 1000 bp from the other end of the fragment.
the presence of 2 HindIII located on opposite sides of the circular molecule, giving rise to two 1000 bp fragments that migrate as a single band.
the presence of many HindIII site, two of which generate a 1000 bp fragment, while the others generate many small fragments that are not revealed by the agarose gel.
the use of an enzyme other the HindIII.
increase.
decrease.
stay about the same regardless of the size of the genome.
will always allow resolution of all of the individual fragments that together comprise the genome.
irrelevant because they do not interfere with accurate generation of restriction maps.
present in enzymes that work in a wide range of digestion conditions and are resistant to digestion artefacts.
the recognition and cleavage of nucleotide sequences larger and more specific than the normal recognition site.
the recognition and cleavage of nucleotide sequences smaller and less specific than the normal recognition site.
once every 4096 bases.
once every 1024 bases.
once every 256 bases.
once every 24 bases.
enzymes with cleavage activity that is inhibited by DNA methylation.
enzymes with large (greater than six bp) recognition sites.
enzymes with small (less than six bp) recognition sites.
those enzymes that are commercially available.
the isolation of the DNA from a host that contains a methylase that modifies the DNA and prevents cleavage by one of the enzymes.
the fact that biological restraints may exclude the use of certain nucleotide sequences in some DNA molecules.
the observation that some nucleotide sequences may occur preferentially in certain DNA sequences.
specificity of recognition and cleavage.
the short cohesive DNA termini generated by digestion.
relative ease of purification and stability of the proteins.
the ability to reseal the termini to make new DNA molecules.
result of the genetic engineering of enzyme specificity by the companies that sell restriction enzymes.
result of the chemical or proteolytic modification of enzyme specificity by the companies that sell restriction enzymes.
due to the wide range of specificity observed in naturally occurring restriction enzymes.